lentiguide puro backbone (Addgene inc)
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lentiguide puro backbone
Lentiguide Puro Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1535 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiguide+puro+backbone/lentiGuide-Puro+(Plasmid+%2352963)/pm42850312-583-8-10
Average 96 stars, based on 1535 article reviews
Lentiguide Puro Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1535 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiguide+puro+backbone/lentiGuide-Puro+(Plasmid+%2352963)/pm42850312-583-8-10
Average 96 stars, based on 1535 article reviews
lentiguide puro backbone - by Bioz Stars,
2026-10
96/100 stars
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Clone Assay:Article Title: The aryl hydrocarbon receptor (AHR) drives human leukocyte antigen (HLA)-II expression in human melanoma. Article Snippet: .. Each sgRNA was cloned into Article Title: Gene targets for T-cell-based immunotherapy Article Snippet: Guides were derived from the Brunello sgRNA library, and the pooled oligo library was ordered from Twist Bioscience. .. Oligos were PCR amplified and cloned into the pgRNA-humanized backbone by Gibson assembly as described by Joung et al. (Joung et al., 2016) For the genome-wide screens, the Brunello plasmid library in the Article Title: The aryl hydrocarbon receptor (AHR) drives human leukocyte antigen (HLA)-II expression in human melanoma Article Snippet: .. Each sgRNA was cloned into Article Title: Dissecting cellular ecosystem with single-cell CRISPR screens Article Snippet: .. A 34-nucleotide sequence, composed of a 4-nt linker and an “8A8G” capture motif, was strategically inserted between the 3′ end of the sgRNA scaffold and the polyT termination signal, and subsequently cloned into the Sequencing:Article Title: The aryl hydrocarbon receptor (AHR) drives human leukocyte antigen (HLA)-II expression in human melanoma. Article Snippet: .. Each sgRNA was cloned into Article Title: The aryl hydrocarbon receptor (AHR) drives human leukocyte antigen (HLA)-II expression in human melanoma Article Snippet: .. Each sgRNA was cloned into Article Title: Dissecting cellular ecosystem with single-cell CRISPR screens Article Snippet: .. A 34-nucleotide sequence, composed of a 4-nt linker and an “8A8G” capture motif, was strategically inserted between the 3′ end of the sgRNA scaffold and the polyT termination signal, and subsequently cloned into the Plasmid Preparation:Article Title: FeaSion decodes the regulatory landscape and functional diversity of RNA polymerase II CTD phosphorylation Article Snippet: After selection, Janelia Fluor 549 HaloTag ligand (Promega, #GA111A) were added to the medium, and monoclonal cells were selected with FACS. .. For KO plasmid construction, we annealed sgRNA oligos into Article Title: Gene targets for T-cell-based immunotherapy Article Snippet: Guides were derived from the Brunello sgRNA library, and the pooled oligo library was ordered from Twist Bioscience. .. Oligos were PCR amplified and cloned into the pgRNA-humanized backbone by Gibson assembly as described by Joung et al. (Joung et al., 2016) For the genome-wide screens, the Brunello plasmid library in the Article Title: Whole-genome CRISPR screening identifies genetic modifiers of stem cell-derived islet transplantation. Article Snippet: Forward and reverse primer (1 μL), T4 ligation buffer (NEB, B0202A), T4 Polynucleotide Kinase (NEB, M0201S), and Ultrapure water were added to a PCR tube and placed in the thermocycler for phosphorylation and annealing reaction. .. The resulting gRNA oligonucleotide duplex was inserted into a Article Title: Alzheimer's disease target and drug discovery by leveraging multiomics and electronic health data. Article Snippet: .. For cloning, sgRNA oligonucleotides were inserted into the Polymerase Chain Reaction:Article Title: Gene targets for T-cell-based immunotherapy Article Snippet: Guides were derived from the Brunello sgRNA library, and the pooled oligo library was ordered from Twist Bioscience. .. Oligos were PCR amplified and cloned into the pgRNA-humanized backbone by Gibson assembly as described by Joung et al. (Joung et al., 2016) For the genome-wide screens, the Brunello plasmid library in the Article Title: PI3K/mTOR activity sensitizes cancer cells to nucleolar stress Article Snippet: A lentiviral vector encoding Cas9, blasticidin resistance, and BFP (Addgene #196714) was transduced, and after blasticidin selection, BFP-high cells were twice sorted by FACS, expanded, and used for the genome-wide CRISPR screen. .. The Brunello sgRNA library ( Doench et al ., 2016 ), targeting 19,114 genes with four sgRNAs per gene and 1,000 non-targeting controls, was resynthesized with Unique Molecular Identifiers (UMIs) ( Schmierer et al , 2017 ) to allow precise PCR-based quantification and amplification. sgRNAs were synthesized as pooled oligos (CustomArray), cloned into a modified Amplification:Article Title: Gene targets for T-cell-based immunotherapy Article Snippet: Guides were derived from the Brunello sgRNA library, and the pooled oligo library was ordered from Twist Bioscience. .. Oligos were PCR amplified and cloned into the pgRNA-humanized backbone by Gibson assembly as described by Joung et al. (Joung et al., 2016) For the genome-wide screens, the Brunello plasmid library in the Article Title: PI3K/mTOR activity sensitizes cancer cells to nucleolar stress Article Snippet: A lentiviral vector encoding Cas9, blasticidin resistance, and BFP (Addgene #196714) was transduced, and after blasticidin selection, BFP-high cells were twice sorted by FACS, expanded, and used for the genome-wide CRISPR screen. .. The Brunello sgRNA library ( Doench et al ., 2016 ), targeting 19,114 genes with four sgRNAs per gene and 1,000 non-targeting controls, was resynthesized with Unique Molecular Identifiers (UMIs) ( Schmierer et al , 2017 ) to allow precise PCR-based quantification and amplification. sgRNAs were synthesized as pooled oligos (CustomArray), cloned into a modified Genome Wide:Article Title: Gene targets for T-cell-based immunotherapy Article Snippet: Guides were derived from the Brunello sgRNA library, and the pooled oligo library was ordered from Twist Bioscience. .. Oligos were PCR amplified and cloned into the pgRNA-humanized backbone by Gibson assembly as described by Joung et al. (Joung et al., 2016) For the genome-wide screens, the Brunello plasmid library in the Cloning:Article Title: Alzheimer's disease target and drug discovery by leveraging multiomics and electronic health data. Article Snippet: .. For cloning, sgRNA oligonucleotides were inserted into the DNA Sequencing:Article Title: Alzheimer's disease target and drug discovery by leveraging multiomics and electronic health data. Article Snippet: .. For cloning, sgRNA oligonucleotides were inserted into the Synthesized:Article Title: PI3K/mTOR activity sensitizes cancer cells to nucleolar stress Article Snippet: A lentiviral vector encoding Cas9, blasticidin resistance, and BFP (Addgene #196714) was transduced, and after blasticidin selection, BFP-high cells were twice sorted by FACS, expanded, and used for the genome-wide CRISPR screen. .. The Brunello sgRNA library ( Doench et al ., 2016 ), targeting 19,114 genes with four sgRNAs per gene and 1,000 non-targeting controls, was resynthesized with Unique Molecular Identifiers (UMIs) ( Schmierer et al , 2017 ) to allow precise PCR-based quantification and amplification. sgRNAs were synthesized as pooled oligos (CustomArray), cloned into a modified Modification:Article Title: PI3K/mTOR activity sensitizes cancer cells to nucleolar stress Article Snippet: A lentiviral vector encoding Cas9, blasticidin resistance, and BFP (Addgene #196714) was transduced, and after blasticidin selection, BFP-high cells were twice sorted by FACS, expanded, and used for the genome-wide CRISPR screen. .. The Brunello sgRNA library ( Doench et al ., 2016 ), targeting 19,114 genes with four sgRNAs per gene and 1,000 non-targeting controls, was resynthesized with Unique Molecular Identifiers (UMIs) ( Schmierer et al , 2017 ) to allow precise PCR-based quantification and amplification. sgRNAs were synthesized as pooled oligos (CustomArray), cloned into a modified |